TTC32 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5C12]

CAT#: TA501341AM

Anti-TTC32 mouse monoclonal antibody, clone OTI5C12 (formerly 5C12), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TTC32 (RC209334, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TTC32. Positive lysates LY423407 (100ug) and LC423407 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TTC32 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501341, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TTC32 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501341, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TTC32 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501341, Dilution 1:50)

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  HEK293T cells transfected with either RC209334 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TTC32 antibody (TA501341), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-TTC32 antibody (TA501341), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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Specifications

Product Data

• Clone Name: OTI5C12
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:50, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TTC32(NP_001008238) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 17.1 kDa
• Gene Name: tetratricopeptide repeat domain 32
• Database Link:
  NP_001008238
  Entrez Gene 130502 Human
  UniProt ID Q5I0X7
• Synonyms: tetratricopeptide repeat domain 32