HID1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5C4]

CAT#: TA501369AM

Anti-C17orf28 mouse monoclonal antibody, clone OTI5C4 (formerly 5C4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C17orf28 (RC206740, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C17orf28. Positive lysates LY403066 (100ug) and LC403066 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-C17orf28 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-C17orf28 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501369, Dilution 1:50)

Specifications

Product Data

• Clone Name: OTI5C4
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C17orf28 (NP_085133) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 88.6 kDa
• Gene Name: HID1 domain containing
• Database Link:
  NP_085133
  Entrez Gene 287822 RatEntrez Gene 283987 Human
  UniProt ID Q8IV36
• Synonyms: C17orf28; DMC1; HID-1