CD5 Mouse Monoclonal Antibody [Clone ID: OTI2B8]

CAT#: TA501376

CD5 mouse monoclonal antibody, clone OTI2B8 (formerly 2B8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (RC206494, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5. Positive lysates LY415443 (100ug) and LC415443 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 1 cell line lysate by using anti-CD5 monoclonal antibody (1:500).

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501376)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501376)

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  Anti-CD5 mouse monoclonal antibody (TA501376) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD5 (RC206494).

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  HEK293T cells transfected with either RC206494 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD5 antibody (TA501376), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-CD5 antibody (TA501376), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI2B8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.4 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.4 kDa
• Gene Name: CD5 molecule
• Database Link:
  NP_055022
  Entrez Gene 921 Human
  UniProt ID P06127
• Synonyms: LEU1; T1

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage