XPNPEP1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1E3]

CAT#: TA501383AM

Anti-XPNPEP1 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3), Biotinylated

Product Images

HEK293T cells were transfected with pCMV6-ENTRY XPNPEP1 (RC203535, Left lane) or the pCMV6-ENTRY control (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XPNPEP1.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-XPNPEP1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

• 

  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-XPNPEP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501383, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-XPNPEP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501383, Dilution 1:50)

• 

  Anti-XPNPEP1 mouse monoclonal antibody (TA501383) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY XPNPEP1 (RC203535).

Show all 5 images

Specifications

Product Data

• Clone Name: OTI1E3
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200 - 1:1000, IHC 1:150, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human XPNPEP1 (NP_065116) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.6 kDa
• Gene Name: X-prolyl aminopeptidase 1
• Database Link:
  NP_065116
  Entrez Gene 170750 MouseEntrez Gene 170751 RatEntrez Gene 7511 Human
  UniProt ID Q9NQW7
• Background: This gene encodes the cytosolic form of a metalloaminopeptidase that catalyzes the cleavage of the N-terminal amino acid adjacent to a proline residue. The gene product may play a role in degradation and maturation of tachykinins, neuropeptides, and peptide hormones. Alternative splicing results in multiple transcript variants.
• Synonyms: APP1; SAMP; XPNPEP; XPNPEPL; XPNPEPL1

Reference Data

• Protein Families: Druggable Genome, Protease