ketohexokinase (KHK) Mouse Monoclonal Antibody [Clone ID: OTI3D1]

CAT#: TA501410S

KHK (Ketohexokinase) mouse monoclonal antibody, clone OTI3D1 (formerly 3D1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KHK (RC202424, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KHK. Positive lysates LY400082 (100ug) and LC400082 (20ug) can be purchased separately from OriGene.

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  Anti-KHK mouse monoclonal antibody (TA501410) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KHK (RC202424).

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  HEK293T cells transfected with either RC202424 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KHK antibody (TA501410), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-KHK antibody (TA501410), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-KHK antibody (TA501410), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

• Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-KHK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501410)

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Specifications

Product Data

• Clone Name: OTI3D1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human KHK(NP_000212) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.84 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.5 kDa
• Gene Name: ketohexokinase
• Database Link:
  NP_000212
  Entrez Gene 3795 Human
  UniProt ID P50053
• Background: This gene encodes ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The product of this gene is the first enzyme with a specialized pathway that catabolizes dietary fructose. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
• Synonyms: ketohexokinase; ketohexokinase (fructokinase)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fructose and mannose metabolism, Metabolic pathways