PANK2 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3G4]

CAT#: TA501419AM

PANK2 mouse monoclonal antibody, clone OTI3G4 (formerly 3G4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PANK2 (RC215676, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PANK2. Positive lysates LY406998 (100ug) and LC406998 (20ug) can be purchased separately from OriGene.

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  Anti-PANK2 mouse monoclonal antibody (TA501419) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PANK2 (RC215676).

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  Flow cytometric Analysis of Jurkat cells, using anti-PANK2 antibody (TA501419), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

Specifications

Product Data

• Clone Name: OTI3G4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PANK2 (NP_705902) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.1 kDa
• Gene Name: pantothenate kinase 2
• Database Link:
  NP_705902
  Entrez Gene 74450 MouseEntrez Gene 296167 RatEntrez Gene 80025 Human
  UniProt ID Q9BZ23
• Background: This gene encodes a protein belonging to the pantothenate kinase family and is the only member of that family to be expressed in mitochondria. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by acyl CoA species. Mutations in this gene are associated with HARP syndrome and pantothenate kinase-associated neurodegeneration (PKAN), formerly Hallervorden-Spatz syndrome. Alternative splicing, involving the use of alternate first exons, results in multiple transcripts encoding different isoforms. [provided by RefSeq]
• Synonyms: C20orf48; HARP; HSS; NBIA1; PKAN

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pantothenate and CoA biosynthesis