mu Crystallin (CRYM) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1B5]

CAT#: TA501433BM

Anti-CRYM (mu Crystallin) mouse monoclonal antibody, clone OTI1B5 (formerly 1B5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYM (RC218123, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CRYM. Positive lysates LY419685 (100ug) and LC419685 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-CRYM monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CRYM mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501433, Dilution 1:50)

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  Anti-CRYM mouse monoclonal antibody (TA501433) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CRYM (RC218123).

Specifications

Product Data

• Clone Name: OTI1B5
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1:200 - 1:1000, IHC 1:300, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CRYM (NP_001879) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.6 kDa
• Gene Name: crystallin mu
• Database Link:
  NP_001879
  Entrez Gene 12971 MouseEntrez Gene 117024 RatEntrez Gene 1428 Human
  UniProt ID Q14894
• Background: Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]
• Synonyms: DFNA40; THBP