Ephrin A2 (EFNA2) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3E3]

CAT#: TA501461AM

EFNA2 (Ephrin A2) mouse monoclonal antibody, clone OTI3E3 (formerly 3E3), Biotinylated

Product Images

Anti-EFNA2 mouse monoclonal antibody (TA501461) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EFNA2 (RC213728).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EFNA2 (RC213728, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EFNA2. Positive lysates LY400546 (100ug) and LC400546 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EFNA2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501461)

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  Flow cytometric Analysis of Jurkat cells, using anti-EFNA2 antibody (TA501461), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

Specifications

Product Data

• Clone Name: OTI3E3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EFNA2 (NP_001396) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.3 kDa
• Gene Name: ephrin A2
• Database Link:
  NP_001396
  Entrez Gene 13637 MouseEntrez Gene 1943 Human
  UniProt ID O43921
• Background: This gene encodes a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm. [provided by RefSeq]
• Synonyms: ELF-1; EPLG6; HEK7-L; LERK-6; LERK6

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance