Cytoplasmic dynein 1 light intermediate chain 1 (DYNC1LI1) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1D1]

CAT#: TA501500BM

Anti-DYNC1LI1 mouse monoclonal antibody, clone OTI1D1 (formerly 1D1), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DYNC1LI1 (RC222010, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DYNC1LI1. Positive lysates LY414168 (100ug) and LC414168 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DYNC1LI1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Anti-DYNC1LI1 mouse monoclonal antibody (TA501500) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DYNC1LI1 (RC222010).

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  HEK293T cells transfected with either RC222010 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DYNC1LI1 antibody (TA501500), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1D1
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DYNC1LI1 (NP_057225) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 56.4 kDa
• Gene Name: dynein cytoplasmic 1 light intermediate chain 1
• Database Link:
  NP_057225
  Entrez Gene 235661 MouseEntrez Gene 252902 RatEntrez Gene 51143 Human
  UniProt ID Q9Y6G9
• Synonyms: DLC-A; DNCLI1; LIC1