LIN7 (LIN7B) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1C9]

CAT#: TA501516AM

Anti-LIN7B mouse monoclonal antibody, clone OTI1C9 (formerly 1C9), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIN7B (RC205598, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIN7B. Positive lysates LY411720 (100ug) and LC411720 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-LIN7B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501516, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LIN7B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501516, Dilution 1:50)

• 

  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LIN7B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501516, Dilution 1:50)

• 

  Anti-LIN7B mouse monoclonal antibody (TA501516) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIN7B (RC205598).

• 

  HEK293T cells transfected with either RC205598 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIN7B antibody (TA501516), and then analyzed by flow cytometry.

Show all 6 images

Specifications

Product Data

• Clone Name: OTI1C9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LIN7B (NP_071448) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22.7 kDa
• Gene Name: lin-7 homolog B, crumbs cell polarity complex component
• Database Link:
  NP_071448
  Entrez Gene 64130 Human
  UniProt ID Q9HAP6
• Synonyms: LIN-7B; MALS-2; MALS2; VELI2

Reference Data

• Protein Families: Druggable Genome