QPRT Mouse Monoclonal Antibody [Clone ID: OTI4E5]

CAT#: TA501519

Anti-QPRT mouse monoclonal antibody, clone OTI4E5 (formerly 4E5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY QPRT (RC202960, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-QPRT. Positive lysates LY402307 (100ug) and LC402307 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-QPRT monoclonal antibody.

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  Anti-QPRT mouse monoclonal antibody (TA501519) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY QPRT (RC202960).

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  HEK293T cells transfected with either RC202960 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-QPRT antibody (TA501519), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4E5
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human QPRT (NP_055113) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.63 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.6 kDa
• Gene Name: quinolinate phosphoribosyltransferase
• Database Link:
  NP_055113
  Entrez Gene 23475 Human
  UniProt ID Q15274
• Background: This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: HEL-S-90n; QPRTase

Reference Data

• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism