QPRT Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1C10]

CAT#: TA501524BM

QPRT mouse monoclonal antibody, clone OTI1C10 (formerly 1C10), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY QPRT (RC202960, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-QPRT. Positive lysates LY402307 (100ug) and LC402307 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-QPRT monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-QPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501524)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-QPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501524)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-QPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501524)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-QPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501524)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-QPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501524)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-QPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501524)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-QPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501524)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-QPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501524)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-QPRT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501524)

• Anti-QPRT mouse monoclonal antibody (TA501524) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY QPRT (RC202960).

• HEK293T cells transfected with either RC202960 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-QPRT antibody (TA501524), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1C10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human QPRT (NP_055113) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.6 kDa
• Gene Name: quinolinate phosphoribosyltransferase
• Database Link:
  NP_055113
  Entrez Gene 23475 Human
  UniProt ID Q15274
• Background: This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: HEL-S-90n; QPRTase

Reference Data

• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism