BTN1A1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI7B5]

CAT#: TA501529BM

BTN1A1 mouse monoclonal antibody, clone OTI7B5 (formerly 7B5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN1A1 (RC223852, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN1A1. Positive lysates LY400653 (100ug) and LC400653 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BTN1A1 monoclonal antibody.

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-BTN1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501529)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-BTN1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501529)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BTN1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501529)

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BTN1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501529)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BTN1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501529)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-BTN1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501529)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BTN1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501529)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BTN1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501529)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BTN1A1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501529)

• Anti-BTN1A1 mouse monoclonal antibody (TA501529) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN1A1 (RC223852).

• HEK293T cells transfected with either RC223852 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTN1A1 antibody (TA501529), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-BTN1A1 antibody (TA501529), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-BTN1A1 antibody (TA501529), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

Show all 15 images

Specifications

Product Data

• Clone Name: OTI7B5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200~500, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BTN1A1 (NP_001723) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.8 kDa
• Gene Name: butyrophilin subfamily 1 member A1
• Database Link:
  NP_001723
  Entrez Gene 100855656 DogEntrez Gene 696 Human
  UniProt ID Q13410
• Background: Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families [provided by RefSeq].
• Synonyms: BT; BTN; BTN1

Reference Data

• Protein Families: Druggable Genome, Transmembrane