Breast cancer suppressor candidate 1 (VWA5A) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3A3]

CAT#: TA501597BM

VWA5A mouse monoclonal antibody, clone OTI3A3 (formerly 3A3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VWA5A (RC212185, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VWA5A. Positive lysates LY402354 (100ug) and LC402354 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-VWA5A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Anti-VWA5A mouse monoclonal antibody (TA501597) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VWA5A (RC212185).

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  HEK293T cells transfected with either RC212185 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VWA5A antibody (TA501597), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI3A3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~1000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VWA5A(NP_055437) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 86.3 kDa
• Gene Name: von Willebrand factor A domain containing 5A
• Database Link:
  NP_055437
  Entrez Gene 67776 MouseEntrez Gene 710024 MonkeyEntrez Gene 4013 Human
  UniProt ID O00534
• Background: May play a role in tumorigenesis as a tumor suppressor. Altered expression of this protein and disruption of the molecular pathway it is involved in, may contribute directly to or modify tumorigenesis.
• Synonyms: BCSC-1; BCSC1; LOH11CR2A