RNF86 (TRIM2) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2B10]

CAT#: TA501606AM

TRIM2 mouse monoclonal antibody, clone OTI2B10 (formerly 2B10), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC229368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2. Positive lysates LY414662 (100ug) and LC414662 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRIM2 monoclonal antibody.

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  HEK293T cells transfected with either RC203947 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIM2 antibody (TA501606), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-TRIM2 antibody (TA501606), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-TRIM2 antibody (TA501606), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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Specifications

Product Data

• Clone Name: OTI2B10
• Applications: FC, WB
• Recommended Dilution: WB 1:1000~2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-100 and 1500-1600 of human SHPRH (NP_775105) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81.3 kDa
• Gene Name: tripartite motif containing 2
• Database Link:
  NP_056086
  Entrez Gene 80890 MouseEntrez Gene 361970 RatEntrez Gene 23321 Human
  UniProt ID Q9C040
• Background: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified. [provided by RefSeq]
• Synonyms: CMT2R; RNF86