Adenylate kinase 5 (AK5) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1G3]

CAT#: TA501651BM

AK5 mouse monoclonal antibody, clone OTI1G3 (formerly 1G3), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK5 (RC222241, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK5. Positive lysates LY403569 (100ug) and LC403569 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-AK5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501651)

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  Flow cytometric Analysis of Hela cells, using anti-AK5 antibody (TA501651), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

Specifications

Product Data

• Clone Name: OTI1G3
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AK5 (NP_777283) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63.2 kDa
• Gene Name: adenylate kinase 5
• Database Link:
  NP_777283
  Entrez Gene 229949 MouseEntrez Gene 26289 Human
  UniProt ID Q9Y6K8
• Background: This gene encodes a member of the adenylate kinase family, which is involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. This member is related to the UMP/CMP kinase of several species. It is located in the cytosol and expressed exclusively in brain. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
• Synonyms: AK6

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Purine metabolism