Protein Kinase D2 (PRKD2) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI5G1]

CAT#: TA501672BM

Anti-PRKD2 (Protein Kinase D2) mouse monoclonal antibody, clone OTI5G1 (formerly 5G1), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKD2 (RC215335, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKD2. Positive lysates LY402555 (100ug) and LC402555 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKD2 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501672, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501672, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501672, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PRKD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501672, Dilution 1:50)

• Anti-PRKD2 mouse monoclonal antibody (TA501672) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKD2 (RC215335).

• HEK293T cells transfected with either RC215335 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKD2 antibody (TA501672), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5G1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PRKD2 (NP_057541) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96.5 kDa
• Gene Name: protein kinase D2
• Database Link:
  NP_057541
  Entrez Gene 101540 MouseEntrez Gene 292658 RatEntrez Gene 484427 DogEntrez Gene 716736 MonkeyEntrez Gene 25865 Human
  UniProt ID Q9BZL6
• Background: The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
• Synonyms: HSPC187; nPKC-D2; PKD2

Reference Data

• Protein Families: Druggable Genome, Protein Kinase