B7-1 (CD80) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3F1]

CAT#: TA501681BM

CD80 mouse monoclonal antibody, clone OTI3F1 (formerly 3F1), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (RC206540, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD80 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501681)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501681)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD80 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501681)

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Specifications

Product Data

• Clone Name: OTI3F1
• Applications: IHC, WB
• Recommended Dilution: WB: 1:200 - 1:1000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CD80(NP_005182) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.3 kDa
• Gene Name: CD80 molecule
• Database Link:
  NP_005182
  Entrez Gene 941 Human
  UniProt ID P33681
• Background: The B-lymphocyte activation antigen B7-1 (formerly referred to as B7) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 (MIM 186760) and CTLA4 (MIM 123890) ligands of T cells. [supplied by OMIM]
• Synonyms: B7; B7-1; B7.1; BB1; CD28LG; CD28LG1; LAB7

Reference Data

• Protein Families: Druggable Genome, Transcription Factors, Transmembrane
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Systemic lupus erythematosus, Toll-like receptor signaling pathway, Type I diabetes mellitus, Viral myocarditis