ARHGAP25 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI12E6]

CAT#: TA501744AM

Anti-ARHGAP25 mouse monoclonal antibody, clone OTI12E6 (formerly 12E6), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARHGAP25 (RC217414, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP25. Positive lysates LY402385 (100ug) and LC402385 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARHGAP25 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501744, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ARHGAP25 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501744, Dilution 1:50)

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  Anti-ARHGAP25 mouse monoclonal antibody (TA501744) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARHGAP25 (RC217414).

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  HEK293T cells transfected with either RC217414 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARHGAP25 antibody (TA501744), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI12E6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ARHGAP25 (NP_055697) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72.3 kDa
• Gene Name: Rho GTPase activating protein 25
• Database Link:
  NP_055697
  Entrez Gene 232201 MouseEntrez Gene 500246 RatEntrez Gene 9938 Human
  UniProt ID P42331
• Background: ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]). [supplied by OMIM]
• Synonyms: HEL-S-308; KAIA0053