RIT2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3F4]

CAT#: TA501757BM

Anti-RIT2 mouse monoclonal antibody, clone OTI3F4 (formerly 3F4), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIT2 (RC205367, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIT2. Positive lysates LY401022 (100ug) and LC401022 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RIT2 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501757, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501757, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501757, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-RIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501757, Dilution 1:50)

• HEK293T cells transfected with either RC205367 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RIT2 antibody (TA501757), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3F4
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RIT2 (NP_002921) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.5 kDa
• Gene Name: Ras like without CAAX 2
• Database Link:
  NP_002921
  Entrez Gene 19762 MouseEntrez Gene 291713 RatEntrez Gene 490475 DogEntrez Gene 694050 MonkeyEntrez Gene 6014 Human
  UniProt ID Q99578
• Background: RIN belongs to the RAS (HRAS; MIM 190020) superfamily of small GTPases (Shao et al., 1999 [PubMed 10545207]). [supplied by OMIM]
• Synonyms: RIBA; RIN; ROC2