EPHX2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2F2]

CAT#: TA501761BM

Anti-EPHX2 mouse monoclonal, clone OTI2F2 (formerly 2F2), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EPHX2 (RC202489, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EPHX2. Positive lysates LY419612 (100ug) and LC419612 (20ug) can be purchased separately from OriGene.

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  Anti-EPHX2 mouse monoclonal antibody (TA501761) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY EPHX2 (RC202489).

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  HEK293T cells transfected with either RC202489 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-EPHX2 antibody (TA501761), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2F2
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human EPHX2 (NP_001970) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 62.4 kDa
• Gene Name: epoxide hydrolase 2
• Database Link:
  NP_001970
  Entrez Gene 2053 Human
  UniProt ID P34913
• Background: This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]
• Synonyms: CEH; SEH

Reference Data

• Protein Pathways: Arachidonic acid metabolism, Metabolic pathways