RND1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI4F12]

CAT#: TA501769AM

RND1 mouse monoclonal antibody, clone OTI4F12 (formerly 4F12), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RND1 (RC205535, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RND1. Positive lysates LY415252 (100ug) and LC415252 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 1 cell line by using anti-RND1 monoclonal antibody (1:200).

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  Western blot analysis of extracts (10ug) from Hela cell line by using anti-RND1 monoclonal antibody (1:200).

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  HEK293T cells transfected with either RC205535 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RND1 antibody (TA501769), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4F12
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RND1 (NP_055285) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.9 kDa
• Gene Name: Rho family GTPase 1
• Database Link:
  NP_055285
  Entrez Gene 27289 Human
  UniProt ID Q92730
• Background: Members of the Rho GTPase family, such as RND1, regulate the organization of the actin cytoskeleton in response to extracellular growth factors (Nobes et al., 1998 [PubMed 9531558]). [supplied by OMIM]
• Synonyms: ARHS; RHO6; RHOS

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Axon guidance