PECR Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI2B2]

CAT#: TA501771AM

Anti-PECR mouse monoclonal antibody, clone OTI2B2 (formerly 2B2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PECR (RC200822, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PECR. Positive lysates LY413023 (100ug) and LC413023 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PECR monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PECR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501771, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PECR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501771, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-PECR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501771, Dilution 1:50)

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  Anti-PECR mouse monoclonal antibody (TA501771) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PECR (RC200822).

• HEK293T cells transfected with either RC200822 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PECR antibody (TA501771), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2B2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PECR (NP_060911) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.4 kDa
• Gene Name: peroxisomal trans-2-enoyl-CoA reductase
• Database Link:
  NP_060911
  Entrez Gene 113956 RatEntrez Gene 478901 DogEntrez Gene 695174 MonkeyEntrez Gene 55825 Human
  UniProt ID Q9BY49
• Background: Participates in chain elongation of fatty acids. Has no 2,4-dienoyl-CoA reductase activity
• Synonyms: DCRRP; HPDHASE; HSA250303; PVIARL; SDR29C1; TERP

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Biosynthesis of unsaturated fatty acids