MTMR14 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI6B6]

CAT#: TA501774BM

Anti-MTMR14 mouse monoclonal antibody, clone OTI6B6 (formerly 6B6), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MTMR14 (RC207732, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MTMR14. Positive lysates LY421457 (100ug) and LC421457 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MTMR14 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-MTMR14 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Anti-MTMR14 mouse monoclonal antibody (TA501774) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MTMR14 (RC207732).

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  HEK293T cells transfected with either RC207732 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MTMR14 antibody (TA501774), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI6B6
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MTMR14 (NP_001070993) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72.0 kDa
• Gene Name: myotubularin related protein 14
• Database Link:
  NP_001070993
  Entrez Gene 97287 MouseEntrez Gene 312634 RatEntrez Gene 484668 DogEntrez Gene 64419 Human
  UniProt ID Q8NCE2
• Background: This gene encodes a myotubularin-related protein. The encoded protein is a phosphoinositide phosphatase that specifically dephosphorylates phosphatidylinositol 3,5-biphosphate and phosphatidylinositol 3-phosphate. Mutations in this gene are correlated with autosomal dominant centronuclear myopathy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 18.
• Synonyms: C3orf29

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Secreted Protein