alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: OTI2A9]

CAT#: TA501782S

AFP (alpha-fetoprotein) mouse monoclonal antibody, clone OTI2A9 (formerly 2A9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AFP (RC206622, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AFP. Positive lysates LY400455 (100ug) and LC400455 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-AFP monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-AFP monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-AFP mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501782)

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  Anti-AFP mouse monoclonal antibody (TA501782) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AFP (RC206622).

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  HEK293T cells transfected with either RC206622 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AFP antibody (TA501782), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-AFP antibody (TA501782), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI2A9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200~500, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AFP (NP_001125) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 66.3 kDa
• Gene Name: alpha fetoprotein
• Database Link:
  NP_001125
  Entrez Gene 403551 DogEntrez Gene 704786 MonkeyEntrez Gene 174 Human
  UniProt ID P02771
• Background: This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq]
• Synonyms: AFPD; FETA; HPAFP

Reference Data

• Protein Families: Druggable Genome, Secreted Protein