PUS7 Mouse Monoclonal Antibody [Clone ID: OTI5F12]

CAT#: TA501815

PUS7 mouse monoclonal antibody, clone OTI5F12 (formerly 5F12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PUS7 (RC203902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PUS7. Positive lysates LY412749 (100ug) and LC412749 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PUS7 monoclonal antibody.

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  Anti-PUS7 mouse monoclonal antibody (TA501815) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PUS7 (RC203902).

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  Flow cytometric Analysis of Hela cells, using anti-PUS7 antibody (TA501815), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-PUS7 antibody (TA501815), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI5F12
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PUS7 (NP_061915) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.9 kDa
• Gene Name: pseudouridine synthase 7
• Database Link:
  NP_061915
  Entrez Gene 78697 MouseEntrez Gene 296751 RatEntrez Gene 100856353 DogEntrez Gene 697463 MonkeyEntrez Gene 54517 Human
  UniProt ID Q96PZ0
• Synonyms: FLJ20485; KIAA1897; MGC17720