PUS7 Mouse Monoclonal Antibody [Clone ID: OTI4H5]

CAT#: TA501823

PUS7 mouse monoclonal antibody, clone OTI4H5 (formerly 4H5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PUS7 (RC203902, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PUS7. Positive lysates LY412749 (100ug) and LC412749 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PUS7 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and PUS7-Knockout HeLa cells (KO, Cat# LC833095) were separated by SDS-PAGE and immunoblotted with anti-PUS7 monoclonal antibody TA501823 (1:1000`). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

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  Flow cytometric Analysis of Hela cells, using anti-PUS7 antibody (TA501823), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-PUS7 antibody (TA501823), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI4H5
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PUS7 (NP_061915) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.54 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74.9 kDa
• Gene Name: pseudouridine synthase 7
• Database Link:
  NP_061915
  Entrez Gene 78697 MouseEntrez Gene 296751 RatEntrez Gene 100856353 DogEntrez Gene 697463 MonkeyEntrez Gene 54517 Human
  UniProt ID Q96PZ0
• Synonyms: FLJ20485; KIAA1897; MGC17720