MICAL1 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1C2]

CAT#: TA501841AM

MICAL1 mouse monoclonal antibody, clone OTI1C2 (formerly 1C2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1. Positive lysates LY402940 (100ug) and LC402940 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MICAL1 mouse monoclonal antibody (TA501841) at 1:150 dilution. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Flow cytometric Analysis of Hela cells, using anti-MICAL1 antibody (TA501841), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1C2
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MICAL1 (NP_073602) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 117.7 kDa
• Gene Name: microtubule associated monooxygenase, calponin and LIM domain containing 1
• Database Link:
  NP_073602
  Entrez Gene 64780 Human
  UniProt ID Q8TDZ2
• Synonyms: MICAL; MICAL-1; NICAL