MICAL1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI4B1]

CAT#: TA501893BM

MICAL1 mouse monoclonal antibody, clone OTI4B1 (formerly 4B1), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MICAL1 (Cat# RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1(Cat# TA501893). Positive lysates LY402940 (100ug) and LC402940 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-MICAL1 monoclonal antibody (1:200).

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MICAL1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MICAL1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MICAL1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-MICAL1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MICAL1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-MICAL1 mouse monoclonal antibody (TA501893) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MICAL1 (RC208308).

• HEK293T cells transfected with either RC208308 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MICAL1 antibody (TA501893), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-MICAL1 antibody (TA501893), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Show all 11 images

Specifications

Product Data

• Clone Name: OTI4B1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MICAL1 (NP_073602) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 117.7 kDa
• Gene Name: microtubule associated monooxygenase, calponin and LIM domain containing 1
• Database Link:
  NP_073602
  Entrez Gene 64780 Human
  UniProt ID Q8TDZ2
• Synonyms: MICAL; MICAL-1; NICAL