OTUB2 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI2E5]

CAT#: TA501974BM

OTUB2 mouse monoclonal antibody, clone OTI2E5 (formerly 2E5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB2 (RC209650, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2. Positive lysates LY402958 (100ug) and LC402958 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC209650 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-OTUB2 antibody (TA501974), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2E5
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human OTUB2 (NP_075601) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.0 kDa
• Gene Name: OTU deubiquitinase, ubiquitin aldehyde binding 2
• Database Link:
  NP_075601
  Entrez Gene 68149 MouseEntrez Gene 314405 RatEntrez Gene 78990 Human
  UniProt ID Q96DC9
• Background: Otubains are deubiquitylating cysteine proteases (DUBs; see MIM 602519) that belong to the ovarian tumor (OTU) protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin (UB; see MIM 191339)-protein bond. [supplied by OMIM]
• Synonyms: C14orf137; OTB2; OTU2

Reference Data

• Protein Families: Protease