XLF (NHEJ1) Mouse Monoclonal Antibody [Clone ID: OTI3E6]

CAT#: TA502004

NHEJ1 (XLF) mouse monoclonal antibody, clone OTI3E6 (formerly 3E6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NHEJ1 (RC203393, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1. Positive lysates LY403031 (100ug) and LC403031 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NHEJ1 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and NHEJ1-Knockout HeLa cells (KO, Cat#LC831412) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1 monoclonal antibody TA502004 (1:200). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502004)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502004)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502004)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502004)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502004)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502004)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502004)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502004)

• Anti-NHEJ1 mouse monoclonal antibody (TA502004) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NHEJ1 (RC203393).

• HEK293T cells transfected with either RC203393 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NHEJ1 antibody (TA502004), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-NHEJ1 antibody (TA502004), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI3E6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NHEJ1 (NP_079058) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.41 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.2 kDa
• Gene Name: non-homologous end joining factor 1
• Database Link:
  NP_079058
  Entrez Gene 79840 Human
  UniProt ID Q9H9Q4
• Background: Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq]
• Synonyms: XLF

Reference Data

• Protein Pathways: Non-homologous end-joining