SAMHD1 Mouse Monoclonal Antibody [Clone ID: OTI3F5]

CAT#: TA502024

SAMHD1 mouse monoclonal antibody, clone OTI3F5 (formerly 3F5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1. Positive lysates LY414526 (100ug) and LC414526 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse tissue by using anti-SAMHD1 monoclonal antibody (1:200).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and SAMHD1-Knockout Hela cells (KO, Cat# LC810344) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1 monoclonal antibody TA502024. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502024)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502024)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502024)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502024)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502024)

• Anti-SAMHD1 mouse monoclonal antibody (TA502024) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1 (RC206013).

• Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody (TA502024), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-SAMHD1 antibody (TA502024), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI3F5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200~500, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SAMHD1(NP_056289) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72 kDa
• Gene Name: SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1
• Database Link:
  NP_056289
  Entrez Gene 56045 MouseEntrez Gene 311580 RatEntrez Gene 485862 DogEntrez Gene 709060 MonkeyEntrez Gene 25939 Human
  UniProt ID Q9Y3Z3
• Synonyms: CHBL2; DCIP; HDDC1; MOP-5; SBBI88