PYCR3 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI8H6]

CAT#: TA502036BM

PYCRL mouse monoclonal antibody, clone OTI8H6 (formerly 8H6), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCRL (RC229141, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCRL. Positive lysates LY411510 (100ug) and LC411510 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PYCRL monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502036)

• Anti-PYCRL mouse monoclonal antibody (TA502036) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCRL (RC203382).

• HEK293T cells transfected with either RC203382 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCRL antibody (TA502036), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-PYCRL antibody (TA502036), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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Specifications

Product Data

• Clone Name: OTI8H6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PYCRL (NP_075566) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.5 kDa
• Gene Name: pyrroline-5-carboxylate reductase 3
• Database Link:
  NP_075566
  Entrez Gene 65263 Human
  UniProt ID Q53H96
• Synonyms: PYCR3

Reference Data

• Protein Pathways: Arginine and proline metabolism, Metabolic pathways