PANK3 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3C4]

CAT#: TA502037BM

PANK3 mouse monoclonal antibody, clone OTI3C4 (formerly 3C4), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PANK3 (RC203696, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PANK3. Positive lysates LY411210 (100ug) and LC411210 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC203696 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PANK3 antibody (TA502037), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-PANK3 antibody (TA502037), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-PANK3 antibody (TA502037), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

Specifications

Product Data

• Clone Name: OTI3C4
• Applications: FC, WB
• Recommended Dilution: WB 1:500, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PANK3 (NP_078870) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.9 kDa
• Gene Name: pantothenate kinase 3
• Database Link:
  NP_078870
  Entrez Gene 79646 Human
  UniProt ID Q9H999
• Background: This gene encodes a protein belonging to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. This family member is expressed most abundantly in the liver. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: DKFZp686O22247; FLJ12899; MGC16863

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pantothenate and CoA biosynthesis