Annexin A3 (ANXA3) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1F11]

CAT#: TA502058BM

ANXA3 (Annexin A3) mouse monoclonal antibody, clone OTI1F11 (formerly 1F11), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ANXA3 (Cat# RC201540, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA3 (Cat# TA502058). Positive lysates LY417500 (100ug) and LC417500 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANXA3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-ANXA3 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Figure from citation: Western blot analysis of ANXA3 protein level by using anti-ANXA3 antibody in CD133+ HCC cell lines. HCC cells, including HepG2, SMMC-7721, and Hep3B cells. Dilution: 1:1000 View Citation

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  Figure from citation: Western Blot of ANXA3 protein level by using anti-ANXA3 antibody in human HCC tissues and nontumorous tissues. Dilution: 1:1000 View Citation

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-ANXA3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ANXA3 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Figure from citation: Representative images of immunohistochemical staining of ANXA3 expression in human liver tissues: (C) negative ANXA3 expression in non-tumorous liver parenchyma and (D) weak, (E) moderate, and (F) strong ANXA3 immunoreactivity in HCC tissues. Dilution: 1:500 View Citation. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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Specifications

Product Data

• Clone Name: OTI1F11
• Applications: IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ANXA3 (NP_005130) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.2 kDa
• Gene Name: annexin A3
• Database Link:
  NP_005130
  Entrez Gene 11745 MouseEntrez Gene 25291 RatEntrez Gene 306 Human
  UniProt ID P12429
• Background: This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. [provided by RefSeq, Jul 2008]
• Synonyms: ANX3

Reference Data

• Protein Families: Stem cell - Pluripotency