DUSP27 (DUPD1) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI7E4]

CAT#: TA502080AM

DUPD1 (DUSP27) mouse monoclonal antibody, clone OTI7E4 (formerly 7E4), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DUPD1 (RC214361, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DUPD1. Positive lysates LY424030 (100ug) and LC424030 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-DUPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502080)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-DUPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502080)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DUPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502080)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-DUPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502080)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DUPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502080)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DUPD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502080)

• HEK293T cells transfected with either RC214361 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DUPD1 antibody (TA502080), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-DUPD1 antibody (TA502080), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-DUPD1 antibody (TA502080), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI7E4
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DUPD1(NP_001003892) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.2 kDa
• Gene Name: dual specificity phosphatase and pro isomerase domain containing 1
• Database Link:
  NP_001003892
  Entrez Gene 338599 Human
  UniProt ID Q68J44
• Synonyms: DUSP27; FMDSP

Reference Data

• Protein Families: Phosphatase