GRHPR Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI4H2]

CAT#: TA502089AM

GRHPR mouse monoclonal antibody, clone OTI4H2 (formerly 4H2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRHPR (RC200963, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRHPR. Positive lysates LY415912 (100ug) and LC415912 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GRHPR monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and GRHPR-Knockout 293T cells (KO, Cat# LC841943 ) were separated by SDS-PAGE and immunoblotted with anti-GRHPR monoclonal antibody TA502089 (1:200`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  HEK293T cells transfected with either RC200963 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRHPR antibody (TA502089), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4H2
• Applications: FC, WB
• Recommended Dilution: WB 1:1000~2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GRHPR (NP_036335) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.5 kDa
• Gene Name: glyoxylate and hydroxypyruvate reductase
• Database Link:
  NP_036335
  Entrez Gene 680021 RatEntrez Gene 9380 Human
  UniProt ID Q9UBQ7
• Synonyms: GLXR; GLYD; PH2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways, Pyruvate metabolism