MYD88 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1A10]

CAT#: TA502116AM

MYD88 mouse monoclonal antibody, clone OTI1A10 (formerly 1A10), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYD88 (RC202253, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYD88. Positive lysates LY432175 (100ug) and LC432175 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-MYD88 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MYD88 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502116)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MYD88 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502116)

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  HEK293T cells transfected with either RC202253 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MYD88 antibody (TA502116), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-MYD88 antibody (TA502116), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-MYD88 antibody (TA502116), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1A10
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MYD88 (NP_002459) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.1 kDa
• Gene Name: MYD88 innate immune signal transduction adaptor
• Database Link:
  NP_002459
  Entrez Gene 4615 Human
  UniProt ID Q99836
• Background: This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: MYD88D

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Apoptosis, Toll-like receptor signaling pathway