Annexin A3 (ANXA3) Mouse Monoclonal Antibody [Clone ID: OTI1A5]

CAT#: TA502123S

ANXA3 (Annexin A3) mouse monoclonal antibody, clone OTI1A5 (formerly 1A5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA3 (RC201540, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA3. Positive lysates LY417500 (100ug) and LC417500 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANXA3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ANXA3-Knockout HeLa cells (KO, Cat# LC812280) were separated by SDS-PAGE and immunoblotted with anti-ANXA3 monoclonal antibody TA502123 (1:200`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-ANXA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502123)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ANXA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502123)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ANXA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502123)

• HEK293T cells transfected with either RC201540 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANXA3 antibody (TA502123), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1A5
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ANXA3 (NP_005130) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.2 kDa
• Gene Name: annexin A3
• Database Link:
  NP_005130
  Entrez Gene 11745 MouseEntrez Gene 25291 RatEntrez Gene 306 Human
  UniProt ID P12429
• Background: This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. [provided by RefSeq, Jul 2008]
• Synonyms: ANX3

Reference Data

• Protein Families: Stem cell - Pluripotency