SNX8 Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI4F8]

CAT#: TA502136AM

SNX8 mouse monoclonal antibody, clone OTI4F8 (formerly 4F8), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNX8 (RC205847, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNX8. Positive lysates LY402251 (100ug) and LC402251 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SNX8 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SNX8 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SNX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502136)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SNX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502136)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SNX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502136)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502136)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-SNX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502136)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SNX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502136)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SNX8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502136)

• Anti-SNX8 mouse monoclonal antibody (TA502136) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNX8 (RC205847).

• HEK293T cells transfected with either RC205847 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNX8 antibody (TA502136), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-SNX8 antibody (TA502136), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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Specifications

Product Data

• Clone Name: OTI4F8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SNX8 (NP_037453) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.4 kDa
• Gene Name: sorting nexin 8
• Database Link:
  NP_037453
  Entrez Gene 29886 Human
  UniProt ID Q9Y5X2
• Synonyms: Mvp1