CALCOCO2 Mouse Monoclonal Antibody [Clone ID: OTI6E8]

CAT#: TA502153S

CALCOCO2 mouse monoclonal antibody, clone OTI6E8 (formerly 6E8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CALCOCO2 (RC203843, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCOCO2 (1:2000).

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  Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY CALCOCO2 (RC203843) using anti-CALCOCO2 antibody (TA502153/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).

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  Flow cytometric analysis of living 293T cells transfected with CALCOCO2 overexpression plasmid (RC203843), Red)/empty vector (PS100001, Blue) using anti-CALCOCO2 antibody (TA502153). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Specifications

Product Data

• Clone Name: OTI6E8
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CALCOCO2 (NP_005822) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.46 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.1 kDa
• Gene Name: calcium binding and coiled-coil domain 2
• Database Link:
  NP_005822
  Entrez Gene 10241 Human
  UniProt ID Q13137
• Background: The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: NDP52