SAMHD1 Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1A1]

CAT#: TA502157BM

SAMHD1 mouse monoclonal antibody, clone OTI1A1 (formerly 1A1), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1. Positive lysates LY414526 (100ug) and LC414526 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SAMHD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Western blot analysis of extracts (10ug) from a mouse tissue by using anti-SAMHD1 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502157)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502157)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502157)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502157)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502157)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502157)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502157)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502157)

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SAMHD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502157)

• Anti-SAMHD1 mouse monoclonal antibody (TA502157) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1 (RC206013).

• HEK293T cells transfected with either RC206013 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SAMHD1 antibody (TA502157), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody (TA502157), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-SAMHD1 antibody (TA502157), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1A1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SAMHD1(NP_056289) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72 kDa
• Gene Name: SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1
• Database Link:
  NP_056289
  Entrez Gene 56045 MouseEntrez Gene 311580 RatEntrez Gene 485862 DogEntrez Gene 709060 MonkeyEntrez Gene 25939 Human
  UniProt ID Q9Y3Z3
• Background: This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. [provided by RefSeq]
• Synonyms: CHBL2; DCIP; HDDC1; MOP-5; SBBI88