SQSTM1 Mouse Monoclonal Antibody [Clone ID: OTI1B2]

CAT#: TA502199

SQSTM1 mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1 (1:500).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody.

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  Western blot analysis of extracts (35ug) from 6 different cell by using anti-SQSTM1 monoclonal antibody (1:500).

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  Immunofluorescent staining of Hela cells using anti-SQSTM1 antibody (TA502199/green, upper left; DAPI/blue, lower left; MERGED, upper right) or Isotype control (MERGED, lower right) (1:100).

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  Immunofluorescent staining of A549 cells using anti-SQSTM1 antibody (TA502199/green, upper left; DAPI/blue, lower left; MERGED, upper right) or Isotype control (MERGED, lower right) (1:100).

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  HEK293T cells transfected with either RC203214 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody (TA502199), and then analyzed by flow cytometry (1:100).

• Flow cytometric Analysis of living Hela cells, using anti-SQSTM1 antibody (TA502199), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

• Flow cytometric Analysis of living A549 cells, using anti-SQSTM1 antibody (TA502199), (Red), compared to an IgG isotype control, (green), and negative control (PBS), (Blue) (1:100).

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Specifications

Product Data

• Clone Name: OTI1B2
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SQSTM1 (NP_003891) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.57 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.5 kDa
• Gene Name: sequestosome 1
• Database Link:
  NP_003891
  Entrez Gene 18412 MouseEntrez Gene 113894 RatEntrez Gene 8878 Human
  UniProt ID Q13501
• Background: This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]
• Synonyms: A170; OSIL; p60; p62; p62B; PDB3; ZIP3

Reference Data

• Protein Families: Druggable Genome, Transcription Factors