Hsp40 (DNAJB1) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI1A12]

CAT#: TA502211AM

DNAJB1 (Hsp40) mouse monoclonal antibody, clone OTI1A12 (formerly 1A12), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB1 (RC201762, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB1. Positive lysates LY401851 (100ug) and LC401851 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DNAJB1 monoclonal antibody.

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  Anti-DNAJB1 mouse monoclonal antibody (TA502211) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB1 (RC201762).

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  HEK293T cells transfected with either RC201762 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DNAJB1 antibody (TA502211), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1A12
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:1000~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DNAJB1 (NP_006136) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37.9 kDa
• Gene Name: DnaJ heat shock protein family (Hsp40) member B1
• Database Link:
  NP_006136
  Entrez Gene 81489 MouseEntrez Gene 361384 RatEntrez Gene 718890 MonkeyEntrez Gene 3337 Human
  UniProt ID P25685
• Synonyms: Hdj1; Hsp40; HSPF1; RSPH16B; Sis1