PACSIN3 Mouse Monoclonal Antibody [Clone ID: OTI4A7]

CAT#: TA502215

PACSIN3 mouse monoclonal antibody, clone OTI4A7 (formerly 4A7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3. Positive lysates LY414084 (100ug) and LC414084 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PACSIN3-Knockout 293T cells (KO, Cat# LC812043) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3 monoclonal antibody TA502215, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  HEK293T cells transfected with either RC204103 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PACSIN3 antibody (TA502215), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4A7
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PACSIN3 (NP_057307) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.55 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.3 kDa
• Gene Name: protein kinase C and casein kinase substrate in neurons 3
• Database Link:
  NP_057307
  Entrez Gene 80708 MouseEntrez Gene 311187 RatEntrez Gene 475984 DogEntrez Gene 713919 MonkeyEntrez Gene 29763 Human
  UniProt ID Q9UKS6
• Background: This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2010]
• Synonyms: SDPIII

Reference Data

• Protein Families: Druggable Genome