PPM1G Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI1C12]

CAT#: TA502232BM

PPM1G mouse monoclonal antibody, clone OTI1C12 (formerly 1C12), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1G (RC213685, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1G. Positive lysates LY406071 (100ug) and LC406071 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPM1G monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  Anti-PPM1G mouse monoclonal antibody (TA502232) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPM1G (RC213685).

Specifications

Product Data

• Clone Name: OTI1C12
• Applications: IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPM1G (NP_817092) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.1 kDa
• Gene Name: protein phosphatase, Mg2+/Mn2+ dependent 1G
• Database Link:
  NP_817092
  Entrez Gene 475703 DogEntrez Gene 5496 Human
  UniProt ID O15355
• Background: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]
• Synonyms: PP2CG; PP2CGAMMA; PPP2CG

Reference Data

• Protein Families: Druggable Genome, Phosphatase