SQSTM1 Mouse Monoclonal Antibody [Clone ID: OTI6A7]

CAT#: TA502239

SQSTM1 mouse monoclonal antibody, clone OTI6A7 (formerly 6A7)

Product Images

Anti-SQSTM1 mouse monoclonal antibody (TA502239) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1 (RC203214).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1 (1:500).

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  HEK293T cells transfected with either RC203214 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SQSTM1 antibody (TA502239), and then analyzed by flow cytometry.

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  Figure from citation: Western Blot of SQSTM1 (p62) protein level by using anti-SQSTM1 antibody in human HEp-2 cell lysates. View Citation

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Specifications

Product Data

• Clone Name: OTI6A7
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SQSTM1 (NP_003891) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 47.5 kDa
• Gene Name: sequestosome 1
• Database Link:
  NP_003891
  Entrez Gene 18412 MouseEntrez Gene 113894 RatEntrez Gene 8878 Human
  UniProt ID Q13501
• Background: This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]
• Synonyms: A170; OSIL; p60; p62; p62B; PDB3; ZIP3

Reference Data

• Protein Families: Druggable Genome, Transcription Factors