LMAN1 Mouse Monoclonal Antibody [Clone ID: OTI1B8]

CAT#: TA502269

LMAN1 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (RC207088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1. Positive lysates LY401709 (100ug) and LC401709 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.

• 

  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and LMAN1-Knockout 293T cells (KO, Cat# LC812175) were separated by SDS-PAGE and immunoblotted with anti-LMAN1 monoclonal antibody TA502269, (1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

Specifications

Product Data

• Clone Name: OTI1B8
• Applications: WB
• Recommended Dilution: WB 1:200~500
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LMAN1 (NP_005561) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.2 kDa
• Gene Name: lectin, mannose binding 1
• Database Link:
  NP_005561
  Entrez Gene 70361 MouseEntrez Gene 116666 RatEntrez Gene 476186 DogEntrez Gene 697449 MonkeyEntrez Gene 3998 Human
  UniProt ID P49257
• Background: The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished. [provided by RefSeq]
• Synonyms: ERGIC-53; ERGIC53; F5F8D; FMFD1; gp58; MCFD1; MR60

Reference Data

• Protein Families: Druggable Genome, Transmembrane