beta Catenin (CTNNB1) Mouse Monoclonal Antibody [Clone ID: OTI12C1]

CAT#: TA502306

CTNNB1 mouse monoclonal antibody, clone OTI12C1 (formerly 12C1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTNNB1 (RC208947, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1. Positive lysates LY419662 (100ug) and LC419662 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CTNNB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and CTNNB1-Knockout Hela cells (KO, Cat# LC810056) were separated by SDS-PAGE and immunoblotted with anti-CTNNB1 monoclonal antibody TA502306. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody (TA811000) as a loading control (1:500).

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  Anti-CTNNB1 mouse monoclonal antibody (TA502306) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTNNB1 (RC208947).

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  HEK293T cells transfected with either RC208947 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CTNNB1 antibody (TA502306), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI12C1
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CTNNB1 (NP_001895) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 85.3 kDa
• Gene Name: catenin beta 1
• Database Link:
  NP_001895
  Entrez Gene 12387 MouseEntrez Gene 84353 RatEntrez Gene 477032 DogEntrez Gene 574265 MonkeyEntrez Gene 1499 Human
  UniProt ID P35222
• Background: The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2009]
• Synonyms: armadillo; CTNNB; MRD19

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Focal adhesion, Leukocyte transendothelial migration, Melanogenesis, Pathogenic Escherichia coli infection, Pathways in cancer, Prostate cancer, Thyroid cancer, Tight junction, Wnt signaling pathway