ATP6V1F Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI6C11]

CAT#: TA502322BM

ATP6V1F mouse monoclonal antibody, clone OTI6C11 (formerly 6C11), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1F (RC210728, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1F. Positive lysates LY418132 (100ug) and LC418132 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ATP6V1F monoclonal antibody.

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  HEK293T cells transfected with either RC210728 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATP6V1F antibody (TA502322), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI6C11
• Applications: FC, WB
• Recommended Dilution: WB 1:200~500, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ATP6V1F (NP_004222) produced in E.coli.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 13.2 kDa
• Gene Name: ATPase H+ transporting V1 subunit F
• Database Link:
  NP_004222
  Entrez Gene 116664 RatEntrez Gene 475199 DogEntrez Gene 702112 MonkeyEntrez Gene 9296 Human
  UniProt ID Q16864
• Background: This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein. [provided by RefSeq]
• Synonyms: ATP6S14; VATF; Vma7

Reference Data

• Protein Pathways: Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection